Toxicological and Epigenetic Studies of Two Types of Ale Beer, Tyrosol and Iso-Alpha Humulone

Although many benefits drawn from beer consumption are claimed, the epidemiological records contradictory with respect to cancer prevention. The purpose of this study was investigate possible health-related activities involving genome safety and ageing processes two types lyophilised ale beers (blond stout), as well their bioactive compounds (tyrosol iso-alpha humulone). A multipurpose trial set in vivo toxicity, antitoxicity, mutagenicity, antimutagenicity, lifespan healthspan assays using Drosophila melanogaster were used. In parallel, several vitro designed cell line HL-60 order establish chemopreventive activity selected substances, where epigenetic modulation DNA methylation changes, clastogenic tumour inhibition growth evaluated. four substances confirmed: blond (LBAB), stout (LSAB), tyrosol humulone neither toxic nor genotoxic. Moreover, all except tyrosol, revealed ability protect individual genomes against oxidative radicals exert antimutagenic genotoxin hydrogen peroxide. With degenerative process indicators healthspan, only compound that did not any influence on life extension Drosophila; LBAB induced a significant D. melanogaster; LSAB its distinctive reduction longevity. showed cytotoxic LBAB, cells. proapoptotic fragmentation or strand breakage observed for both at different concentrations. Furthermore, exhibited an increasing genome-wide status, while demethylation status repetitive sequences. biological assigned cannot be linked specific molecule/element due complexity phenolic profile, multifactor brewing process, results obtained let us propose safe potential nutraceutical beverages when consumed moderate amounts. prevention toxicity genetic damage, induction tumor death key shown present research.